Nitrogen fertilization of intensively used grassland aims at maximising yield and optimizing quality. However, future fertilization strategies have to take limited resources and the aim of reducing negative side effects, such as gaseous (N2O, NH3, NOx) and liquid N losses (NO3-) into consideration. To reach this aim and apply the right amount of fertilizer, it is necessary to be able to predict the biomass development and N uptake in grassland.